Laboratory of Mass Spectrometry and Gaseous Ion Chemistry National Center of Research Resources National Resource for the Mass Spectrometric
Analysis of Biological Macromolecules
Brian T. Chait
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D. Fenyö, Proteomics Informatics Workshop 2011

An overview of proteomics and mass spectrometry workflows, experimental design, and data analysis.

Part I: Protein identification (peptide mass fingerprinting, tandem mass spectrometry, database searching, spectrum library searching, de novo sequencing, significance testing). (Slides*)

Part II: Protein characterization (protein coverage, top-down proteomics, post-translational modifications, protein processing and degradation, protein complexes). (Slides*)

Part III: Protein quantitation (metabolic labeling - SILAC, chemical labeling, label-free quantitation, spectrum counting, stoichiometry, biomarker discovery and verification) Examples will be provided throughout the course on how the different approaches can be applied to investigate biological systems. (Slides*)

*Audio accompaniment for the slides is in preparation.