Laboratory of Mass Spectrometry and Gaseous Ion Chemistry
PROWL
ProFound
ProteinInfo
PeptideMap
X! Tandem
X! Hunter
GPMDB
   
PROWL
Chait Lab

The Rockefeller University
The Rockefeller University
1230 York Avenue,
New York, NY 10021
(212) 327-8000


National Center of Research Resources
National Resource
for the Mass Spectrometric
Analysis of Biological
Macromolecules

PeptideMap
Name
Protein Sequence
 


CLEAVAGE CHEMISTRY
 
Pre-Defined Enzymatic Cleavages
 
Enzyme
Allow maximum  missed cleavages
Enzyme
Allow maximum  missed cleavages


User-Defined Enzymatic Cleavages
 
Table of User-Defined Enzymes
Amino Acid Terminus C     N
Allow up to  missed cleavages
Chemical Modification
No cleavage near Proline
Cleavage Site(s) Amino Acid Terminus Max. No. Missed Cleavages Chemical Modification Cleavage near Proline


GLOBAL CHEMICAL MODIFICATIONS
 
Pre-Defined Global Modifications
 
Complete Modifications
Partial Modifications

User-Defined Global Modifications
 
User-Defined Global Modifications:
Amino Acid Site(s)
Chemical Formula
Minimal Occupancy
Maximal Occupancy
Chemical Cleavage Site(s) Modification Formula Minimal Occupancy Maximal Occupancy
 


LOCAL CHEMICAL MODIFICATIONS
 
User-Defined Local Modifications
 
User-Defined Local Modifications:
Protein Unit  (Protein units starts at 0)
Amino Acid Position  (Amino acids starts at 1)
Chemical Formula
Minimal Occupancy
Maximal Occupancy
Protein Unit Amino Acid Position Modification Formula Minimal Occupancy Maximal Occupancy
 


CROSSLINKING
 
Pre-Defined Crosslinking
Type of Crosslinking

User-Defined Crosslinking
Cross Linking Reagent
 
N-Terminal as Crosslinking Site
(such as in Lysine)
Max. number of
Crosslinked Fragments
Max. Number of Crosslinks
Link Site(s) 1
Modification 1
Link Site(s) 2
Modification 2


MASSES
 
Input Type Mass     M/z [M+H]+
 
Measured Values in Mass [M] (in mass units u) or Mass-to-Charge Ratios [M+H+] (in Th):
Average Values
Tolerance  ±  in u (≈Da)/Th % ppm
Monoisotopic Values
Tolerance  ±  in u (≈Da)/Th % ppm