Research Projects

The Molecular Architecture of the Nuclear Pore Complex

 

Nuclear pore complexes (NPCs) are proteinaceous assemblies of approximately 50MDa that selectively transport cargoes across the nuclear envelope. To determine the molecular architecture of the yeast NPC, we collected a diverse set of biophysical and proteomic data, and developed a method for using these data to localize the 456 constituent proteins of the NPC. (See video of the structure of the nuclear pore complex.)

 

 

 

References:

Alber, F.; Dokudovskaya, S.; Veenhoff, L. M.; Zhang, W.; Kipper, J.; Devos, D.; Suprapto, A.; Karni-Schmidt, O.; Williams, R.; Chait, B. T.; Sali, A.; Rout, M. P. The molecular architecture of the nuclear pore complex. Nature, v.450, pp.695-701, 2007.

Alber, F.; Dokudovskaya, S.; Veenhoff, L. M.; Zhang, W.; Kipper, J.; Devos, D.; Suprapto, A.; Karni-Schmidt, O.; Williams, R.; Chait, B. T.; Rout, M. P.; Sali, A. Determining the architectures of macromolecular assemblies Nature, v.450, pp.683-694, 2007.

 

Supplementary data: http://www.salilab.org/npc.