Highlights

A new approach gives researchers the ability to isolate single antibodies as well as investigate entire families of highly active antibodies against HIV. more...

A high-capacity ion trap (>10⁶ ions) is coupled to a TOF mass spectrometer

A high-capacity ion trap coupled to a time-of-flight (TOF) mass spectrometer has been developed to carry out comprehensive linked scan analysis of all stored ions in the ion trap. The approach involves a novel tapered geometry high-capacity ion trap that can store more than 106 ions (range 800–4000 m/z) without degrading its performance. Ions are stored and scanned out from the high-capacity ion trap as a function of m/z, collisionally fragmented and analyzed by TOF. more: read paper or see video.

Webcasts of lab presentations at ASMS 2010

The presentations discuss lossless mass spectrometry, MRM interference, and replication fork dynamics. more...

Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome

Prior studies in budding yeast have led to a picture wherein the replication of DNA progresses at variable rates over different parts of the genome. Here, time-resolved ChIP-chip analysis and computer simulations lead to a new picture in which replication fork progression is remarkably uniform throughout the genome. more...

Research suggests core nuclear pore elements shared by all eukaryotes

About 1.7 billion years ago, the cell nucleus burst onto the scene, sequestering the cell’s genetic material inside a protective inner membrane and setting the stage for the evolution of increasingly sophisticated creatures from yeast, say, to plants and human beings. Now research shows that one of the most basic design principles of this new eukaryotic life-form - the gatekeeper to the cell nucleus known as the nuclear pore complex - is largely shared across even the most distantly related eukaryotes. more...

New sequencing technique to prod medical benefits from killer venom

King Kong toxin, a component of the venom in some poisonous marine snails, has a peculiar power to go with its peculiar name. When injected into a meek little lobster in a tank full of superiors, the poison induces delusions of grandeur. more...

Researchers construct a device that mimics one of nature’s key transport machines

A cell’s nuclear pore complex controls transport of materials from one side to the other. Now, by trying to recreate the structure, scientists have gained a broader understanding of how it works. more...

The molecular architecture of the nuclear pore complex

Nuclear pore complexes (NPCs) are proteinaceous assemblies of approximately 50 MDa that selectively transport cargoes across the nuclear envelope. To determine the molecular architecture of the yeast NPC, we collected a diverse set of biophysical and proteomic data, and developed a method for using these data to localize the NPC's 456 constituent proteins. more...